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| Title | Fundamentals of Fluorescence Microscopy : Exploring Life with Light |
| Author(s) | Mondal, Partha Pratim;Diaspro, Alberto |
| Publication | Dordrecht, Springer Netherlands, 2014. |
| Description | XV, 218 p. 140 illus., 55 illus. in color : online resource |
| Abstract Note | This book starts at an introductory level and leads reader to the most advanced developments in fluorescence imaging and super-resolution techniques that have enabled the emergence of new disciplines such as nanobioimaging, multiphoton microscopy, photodynamic therapy, nanometrology and nanosensors. The interdisciplinary subject of fluorescence microscopy and imaging requires complete knowledge of imaging optics and molecular physics. So, this book approaches the subject by introducing optical imaging concepts before going deep into the advanced imaging systems and their applications. Molecular orbital theory forms the basis for understanding fluorescent molecules and thereby facilitates complete explanation of light-matter interaction at the geometrical focus. The two disciplines have some overlap since light controls the states of molecules and conversely, molecular states control the emitted light. These two mechanisms together determine essential fluorescence factors and phenomena such as, molecular cross-section, Stokes shift, emission and absorption spectra, quantum yield, signal-to-noise ratio, Forster resonance energy transfer (FRET), fluorescence recovery after photobleaching (FRAP) and fluorescence lifetime. These phenomena form the basis of many fluorescence based devices. The book is organized into two parts. The first part deals with basics of imaging optics and its applications. The advanced part covers many imaging techniques and related instrumentation that are developed in the last decade pointing towards far-field diffraction limited and unlimited imaging |
| ISBN,Price | 9789400775459 |
| Keyword(s) | 1. Biological and Medical Physics, Biophysics
2. Biological Microscopy
3. BIOLOGICAL PHYSICS
4. BIOPHYSICS
5. EBOOK
6. EBOOK - SPRINGER
7. LASERS
8. MICROSCOPY
9. Optics, Lasers, Photonics, Optical Devices
10. PHOTONICS
11. SPECTROSCOPY
12. Spectroscopy and Microscopy
13. Spectroscopy/Spectrometry
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| Item Type | eBook |
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Circulation Data
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Call# | Status | Issued To | Return Due On | Physical Location |
| I06587 |
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